Lab4 probiotics and the Cholesterol Study

The anti-cholesterolaemic effect of a consortium of probiotics: An acute study in C57BL/6J mice (Part 1)

Aim

To examine the cholesterol lowering abilities of Lab4 probiotics in an in vitro model of the intestinal epithelium.

Method

  • Cholesterol lowering ability and bile salts hydrolase (BSH) activity in Lab4 probiotics was assessed using in vitro models
  • The effect of Lab4 probiotics on cholesterol transport was assessed in an in vitro Caco-2 cells intestinal model

Results

1. Cholesterol removal

  • Cholesterol levels in bacterial culture media were lowered in the presence of Lab4 probiotic mixture (#P=0.076)

 2. Bile salt hydrolase activity

  • A white precipitate was observed when Lab4 probiotic mixture was grown in the presence of bile salts, which is indicative of BSH activity.
BSH activity

 3. Cholesterol transport

  • Lab4 probiotic mixture significantly reduced the expression of cholesterol transporter NPC1L1, which is critical for the uptake of cholesterol (**P<0.001)
  • Lab4 probiotic mixture significantly reduced the efflux of intracellular cholesterol from intestinal epithelial cells into the basolateral (tissue) compartment (*P=0.004)
  • Lab4 probiotic mixture significantly reduced the expression of cholesterol transporter ABCA-1, which is involved in the efflux of intracellular cholesterol to the basolateral (tissue) compartment (*P<0.01)

Summary

Lab4 probiotics Mechanism of Action 

1. Cholesterol Removal

[Cholesterol is bound or metabolised by Lab4 and subsequently excreted in faeces]

2. Bile salt hydrolase activity

[deconjugation (modifying) of bile acids leads to their reduced re-absorption and places an increased demand on the liver to synthesize more bile acids from circulating cholesterol in blood to replenish intestinal bile acids lost in faeces]

3. Reduction of cholesterol transport across the intestinal epithelium

[3a – reduced expression of the NCP1L1 cholesterol transporter; 3b- reduction in the efflux of intracellular cholesterol to the basolateral compartment (tissue) and reduced expression of the ABCA-1 cholesterol transporter; 3c – no change on the intracellular cholesterol efflux back into the apical (intestinal lumen) compartment]

Conclusion

Lab4 probiotics showed promising cholesterol lowering potential.

Reference

Michael DR et al. The anti-cholesterolaemic effect of a consortium of probiotics: An acute study in C57BL/6J mice.
Scientific Reports 2017: 7:2883