Lactobacillus plantarum CUL66 and the Cholesterol Study

Lactobacillus plantarum CUL66 can impact cholesterol homeostasis in Caco-2 enterocytes

Aim

To examine the cholesterol lowering ability of Lactobacillus plantarum CUL66 in an in vitro model of the intestinal epithelium.

Method

  • Cholesterol lowering ability and bile salts hydrolase (BSH) activity in L. plantarum CUL66 was assessed using in vitro models
  • The effect of L. plantarum CUL66 on cholesterol transport was assessed in an in vitro Caco-2 cell intestinal model

Results

1. Cholesterol removal

  • Cholesterol levels in bacterial culture media were lowered in the presence of L. plantarum CUL66 (**P<0.001)

 2. Bile salt hydrolase activity

  • A white precipitate was observed when L. plantarum CUL66 was grown in the presence of bile salts, which is indicative of BSH activity
BSH activity

 3. Cholesterol transport

  • L. plantarum CUL66 significantly reduced the uptake of extracellular cholesterol by intestinal epithelial cells (**P<0.001)
  • L. plantarum CUL66 significantly reduced the expression of cholesterol transporter NPC1L1, which is critical for the uptake of cholesterol (*P=0.015)
  • L. plantarum CUL66 significantly reduced the efflux of intracellular cholesterol from intestinal epithelial cells into the basolateral (tissue) compartment (**P<0.001)
  • L. plantarum CUL66 significantly reduced the expression of cholesterol transporter ABCA-1, which is involved in the efflux of intracellular cholesterol to the basolateral (tissue) compartment (**P<0.001)

Summary

Lactobacillus plantarum CUL66 Mechanism of Action

1. Cholesterol Removal

[Cholesterol is bound or metabolised by CUL66 and subsequently excreted in faeces.]

2. Bile salt hydrolase activity

[Deconjugation (modifying) of bile acids leads to their reduced re-absorption and places an increased demand on the liver to synthesize more bile acids from circulating cholesterol in blood to replenish intestinal bile acids lost in faeces.]

3. Reduction of cholesterol transport across the intestinal epithelium 

[3a – reduction in the uptake of extracellular cholesterol and reduced expression of the NCP1L1 cholesterol transporter; 3b- reduction in the efflux of intracellular cholesterol to the basolateral compartment (tissues) and reduced expression of the ABCA-1 cholesterol transporter; 3c – no change on the intracellular cholesterol efflux back into the apical (intestinal lumen) compartment.]

Conclusion

Lactobacillus plantarum CUL66 is a very promising cholesterol lowering candidate.

Reference

Michael DR et al. Lactobacillus plantarum CUL66 can impact cholesterol homeostasis in Caco-2 enterocytes.
Beneficial Microbes 2016; 7(3):443-51