The Cardiff Tracking Study
Detection of Lactobacillus salivarius CUL61 and Lactobacillus acidophilus CUL21 in faecal samples of a healthy volunteer on day 7:
Image (A) shows L. salivarius CUL61 colonies from faecal sample grown on plate. The colonies are labelled as No. 6 and No. 7
Image (B) shows L. acidophilus CUL21 colonies from faecal sample grown on plate. These colonies are labelled as Nos. 3, 4 and 5
Image (C) shows the RAPD fingerprint technique results, as follows:
Lanes 6, 7 – the fingerprint of the L. salivarius CUL61 from colonies in the faecal samples are the same as the original L. salivarius CUL61 control (Lane 1)
Lanes 3, 4, 5 – the fingerprint of the L. acidophilus CUL21 from colonies in the faecal samples are the same as the original L. acidophilus CUL21 control (Lane 2)
Lane numbers correspond to the number labels for the colonies in the faecal samples. M is the marker column that checks that the reactions work. Lane 1 is the fingerprint for the original control for L. salivarius. Lane 2 is the control for L. acidophilus. Lanes 1 and 2 are the fingerprint of the bacterial DNA for the original pure cultures from which the probiotic strains for the capsules are produced.